Contact us

Binding

Home » Main product » Binding

Quantitative Analysis of Target–ligand interactions by CSP NMR titrations

A powerful tool that automatically processes 1D and 2D NMR Target-ligand titration spectra, tracks peaks movement, generates the binding curves and calculates values for 1:1 complexes. For a more sophisticated analysis, the binding curves are directly exported to the 3rd party software AFFINImeter, allowing global analysis to a variety of binding models. .

Download Mnova for a 45-Day Free Trial on Mnova Binding

Mnova Binding includes a FREE 1-year license of AFFINImeter for NMR


AFFINImeter for Nuclear Magnetic Resonance allows the advanced analysis of binding isotherms from 2D NMR titrations to measure binding constants (KA). The binding isotherms are imported directly from the software Mnova and a series of advanced tools are available in AFFINImeter-NMR to get the most out of your NMR data including:

  • Global analysis of binding isotherms.
  • Analysis with complex binding model.
  • Advanced algorithms to guarantee convergence avoiding local minima, as well as to assess the parameter uncertainties, the goodness of fit and result reliability.
Binding

Benefits

Intuitive workflow that allows you to analyze CSP titration experiments interactively or fully automatically.

Automatic peak tracking, binding curve generation and fitting to compute dissociation constant (Kd) of 1:1 complexes.

Easy exporting of binding curves to the 3rd party software AFFINImeter for advanced analyses, e.g, global analysis of a set of titration curves and availability of a variety of binding models.

Binding

Features

Automated peak tracking and binding curve generation

Automated peak tracking across the titration spectra, with manual refinement also available.

The figure shows an example of a peak tracked with its corresponding CSP values and the resulting binding curve, demonstrating the accuracy and efficiency of the automated process in analyzing binding interactions.

Automated individual/average Kd calculation

Automated calculation of the dissociation constant (Kd) for each tracked peak is performed upon generation of the binding curve. This feature also includes the automatic calculation of the average Kd from the set of individual Kd values calculated for each peak.

This process streamlines the determination of binding affinities, ensuring accurate and consistent results while saving valuable time for researchers.

Fully automated analysis

A series of titrations can be specified simply by listing them sequentially in the “titration file”. The names for the ligands used are coded in the “ligands file”. When CSP runs the analysis automatically, it creates different Mnova files for each titration listed in the “titration file,” and the Mnova documents are named according to the ligands’ names.

This feature ensures a seamless and efficient workflow, eliminating manual input and reducing errors, thus enhancing the overall productivity of the analysis process

Binding

Videos

Help & Resources

Guides & Manuals

Mnova Binding - Starting guide

Videos

Mnova Binding - Interactive Mode Mnova Binding - Batch Processing Mode

Publications

NMR characterization of the interaction between Bcl-xL and...
Binding

Related Applications

Academic, Government & Industrial

Target Audience

Medicinal Chemists
Biochemists
Anaytical Chemists
Features

Related Products

0
This site is registered on wpml.org as a development site. Switch to a production site key to remove this banner.