Preparative chromatography is crucial for achieving high-purity materials, particularly challenging with chiral compounds. Separating enantiomers and diastereoisomeric impurities is essential to ensure target species purity. These screenings inform decisions on synthetic routes, preparative methods, and alternative separation techniques. In this application note, Prof. Rafael Cela and Dr. Leticia Pérez-Mayán from Mestrelab Research Center (CIM) outline the use of Mgears Chrom Best Method for evaluating LC separation of diniconazol, showcasing its effectiveness in meeting these analytical demands.